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Protists are difficult to classify, and classification has changed over time. Modern definitions of protists are: "any eukaryote that is not an animal, (land) plant, or (true) fungus" and the second, which describes them mostly by functional or biological terms: "eukaryotes that are never multicellular, that either exist as independent cells, or if they occur in colonies, and do not show differentiation into tissues."

Modern classification uses monophyletic groups that are based on ultrastructure, biochemistry, and genetics.

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