Glyconeogenesis is the metabolic process through which glucose is synthesized from non-carbohydrate precursors, such as lactate, glycerol, and amino acids. This pathway primarily occurs in the liver and, to a lesser extent, in the kidneys. It serves to maintain blood glucose levels during fasting or intense exercise when glycogen stores are depleted. Glyconeogenesis is essentially the reverse of glycolysis, involving several unique enzymes to bypass irreversible steps in glycolysis.