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Macconkey agar consists of agar, lactose, peptone, neutral red dye, crystal violet dye & bile salts. Bile salts inhibit growth of Gram positive bacteria & allow growth of Gram negative bacteria only. It differentiates lactose fermentating Gram negative bacteria from non lactose fermentating Gram negative bacteria.
Wiki User
∙ 13y ago
Wiki User
∙ 9y agoAlfred Theodore MacConkey, M.D developed MacConkey agar at the turn of the 20th century. It was the first solid differential media to be formulated.
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Crystal violet in the MacConkey agar?
MacConkey's agar refers to a culture medium that is designed to selectively grow Gram-negative bacteria. It contains crystal violet dye, which inhibits certain Gram-positive bacteria.
How do you determine when a loop is cool?
Touch it to the sterile agar in a plate. If it melts the agar, it is still hot. If it doesn't melt the agar, then it is cool enough.
What is melting point of agar?
85C
In the diffusion of liquids through agar what characteristic of liquid determines the rate at which it will travel through the agar?
The size of the molecules.
What is non selective media?
Non-selective is when we use agar for bacterial growth in general. Mean not to differentiate between them but just to see their colonies and growth. TSA and Chocolate agar are nonselective.
What type of medium is macconkey agar?
types macconkey agar
What is a Mac plate?
MacConkey Agar plate
Is macconkey agar a synthetic media?
yes
Is macConkey agar defined or undefined?
Undefined.
How do you grow Klebsiella pneumoniae?
MacConkey agar
Why MacConkey agar is solid at room temperature while MacConkey broth remains Liquid at room Temperature?
MacConkey agar is solid at room temperature because it is a gel-like polysaccharide. Broth that has 1.5 percent agar in it and is heated up will become a liquid.
What is the name of the acide base color indicator found in MacConkey agar?
Lac+ bacteria is acid base color indicator that lowers the pH of the MacConkey agar. The MacConkey agar contains neutral red dye, lactose, peptone, and crystal violet dye.
How does yersinia look on nutrient agar EMB agar and MacConkey agar?
Colorless, it doesnt ferment lactose.
Does enterococcus faecalis grow on macConkey agar?
this MacConkey with crystal violet can grow enterococcus
What is the culture characteristic of Ecoli thst grow on macconkey agar?
E.coli shows pink colour colonies in MacConkey agar due to fermentation of lactose
Does micrococcus luteus grow on macconkey agar?
MacConkey agar is designed to grow Gram-negative bacteria and also contains crystal violet dye which inhibits the growth of Gram-positive bacteria. Since S. aureus is Gram-positive it should not grow on MacConkey agar. No. MacConkey agar inhibits MOST of the Gram-positive bacteria. There are some Gram-positive bacteria that can tolerate bile-salt are able to grow in MacConkey agar. Enterococcus and some species of Staphylococcus are able to grow in MacConkey. (lack citation though)
Will Alcaligenes faecalis grow on MacConkey agar?
yes
