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Dextran is are multibranched polysaccharides found to bind with proteins. In this case sephadex we are seeing a brand name for a product made out of this sugar by GE Healthcare. They are used in Size Exclusion Chromatography... (i.e. they run a bunch of fluid through a tube full of these beads and a buffer). The largest proteins flow right on through, the smaller ones get bound up in the beads. If they know the size of the protein they are looking for they can regulate the size of the bead pores to help, and also the chromatography machine has a built in spectrophotometer set at 280 nm (size at which most of your amino acids are reflecting UV- aromatics especially)... so...
simply it is the brand name of bead made of sugar used in a chromatography machine made by GE.
What else can I help you with?
Is dextrin the same as dextran?
No, dextrin and dextran are not the same. Dextrin is a short-chain carbohydrate produced from starch, while dextran is a long-chain carbohydrate produced by bacteria during fermentation. They differ in structure, properties, and uses.
What is the pore size of sephadex g-100?
Sephadex G-100 has a pore size that typically ranges from 40 to 100 nanometers. This makes it suitable for the separation of molecules such as proteins and polysaccharides, allowing larger molecules to elute first while smaller molecules are retained within the gel matrix. It is commonly used in size exclusion chromatography for the purification and analysis of biomolecules.
What is an impermeable carbohydrate that is often administered to patients suffering blood loss?
Dextran is an impermeable carbohydrate commonly administered to patients with blood loss, as it helps expand blood volume by drawing fluid into the bloodstream.
Can amylase break down dextran?
Yes, amylase can break down dextran. Amylase is an enzyme that primarily breaks down starch into simpler sugars like maltose and glucose. Dextran is a complex sugar made of glucose molecules linked together, and amylase can break the bonds holding these glucose molecules together, albeit less efficiently compared to starch.
What are the uses of dextran?
Dextran is commonly used as a plasma volume expander in medical settings to help increase blood volume. It is also used in chromatography techniques to separate biomolecules based on their size or charge. Additionally, dextran can be incorporated into drug delivery systems to improve drug stability and bioavailability.
How dextran can be analyzied in sugar cane juice and final molasses?
Dextran in sugar cane juice and final molasses can be analyzed using high-performance liquid chromatography (HPLC) coupled with a refractive index detector or a size exclusion chromatography (SEC) method. These techniques separate dextran based on molecular weight and enable quantification by comparing the sample's response to that of known standards. Additionally, spectrophotometric methods may be employed to assess total carbohydrate content, allowing for indirect estimation of dextran levels. Proper sample preparation and calibration are essential for accurate results.
What has the author SJ Sibson written?
S.J Sibson has written: 'Solvent extraction of dextran from fermentation products'
What does soluble starch mean?
Soluble starch is a partially hydrolyzed (mostly by acid hydrolysis) starch, therefore it actually is a long-chain dextran.
What has the author Keith Robert Poland written?
Keith Robert Poland has written: 'The fractionation of dextran polymer by ultrafiltration to yield clinical products'
What two drugs which must be given in the Z track method?
Any medication that is brown colored or will discolor the skin/ or will be extremely irritating to the resident.
Dextran is what type of fluid for shock?
Dextran is a colloid solution used in the treatment of shock, particularly for volume resuscitation in cases of hypovolemia. As a synthetic polysaccharide, it helps to expand plasma volume and improve blood circulation by increasing oncotic pressure. It is often utilized when crystalloids are insufficient, particularly in certain surgical or trauma contexts. However, its use may be limited due to potential side effects, including allergic reactions and effects on blood coagulation.
How do they extract the stem cells from umbilical cord blood?
They extract the stem cells from umbilical cord blood in albumin or dextran before infusion into patients. You can read more about it at www.cordblood.com/cord_blood_faqs/cord_tissue.asp.
