Coagulation studies-Prothrombin Time (PT), Coumadin therapy, Partial Thromboplastin Time (APTT, PTT), Thrombin Time (TT) Vleeding time (BT)
blue
In phlebotomy, if an orange shield tube is collected before a light blue stopper tube, it can lead to inaccurate results in coagulation tests. The orange tube typically contains a gel separator for serum testing, which may contaminate the blue tube's citrated plasma sample, potentially affecting the measurement of clotting factors. To ensure accurate results, the light blue tube should always be collected first when performing tests that require it.
Drawing a discard tube before a blue tube in blood collection prevents contamination with tissue fluids or substances that could affect test results. The discard tube helps clear the line of air and ensure that the blood sample collected in the blue tube is free from any potential contaminants.
Orange shield tube is usual for infectious disease tests. Blue top may be used for many types of test.
If an orange shield tube is collected before a light blue shield tube, it may lead to sample contamination or interference in the results of certain blood tests. The order of collection is crucial to prevent cross-contamination of additives, which can affect the accuracy of test results. Additionally, certain tests may require specific tubes to be collected first to ensure proper specimen handling. Following the recommended order of draw is essential for reliable laboratory results.
Platelet function studies are typically collected in a blue-top tube (sodium citrate) to prevent clotting and maintain the function of platelets for analysis.
A lavender-top tube should be collected for a complete blood count (CBC).
Clotting
The haptoglobin test is typically collected in a red-top tube, which does not contain any additives. Alternatively, it can also be collected in a serum separator tube (SST).
A red top tube (serum separator tube) is typically used to collect blood for digoxin level testing. The tube should be inverted gently to mix the blood with the anticoagulant and clot activator present in the tube. Once the sample is collected, it should be processed promptly to separate the serum for analysis.
If a lavender tube is collected before a light green tube, there is a risk of contamination and carryover of additives present in the lavender tube into the sample collected in the light green tube. This can affect the accuracy of test results and potentially lead to erroneous findings. It is important to follow the correct order of draw to ensure the integrity of the samples.
Blood for CBC is usually collected in a purple-top tube (containing EDTA).