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Which enzyme is used to produce RFLPs?
Restriction enzymes.
The use of RFLPs for genetic fingerprinting is based on?
The use of RFLPs in generic fingerprinting is based on the ability of restriction enzymes to dissect DNA into small fragments. There are many kinds of restriction enzymes made to cut various DNA sequences.
How are RFLPs detected?
by the guard dog
Which enzyme is used to produce RFLPs?
Restriction enzymes.
What are cutting DNA at specific sites to form restriction fragments called?
RFLPs
The use of RFLPs for genetic fingerprinting is based on?
The use of RFLPs in generic fingerprinting is based on the ability of restriction enzymes to dissect DNA into small fragments. There are many kinds of restriction enzymes made to cut various DNA sequences.
What is RFLP used for?
RFLP (often pronounced "rif lip", as if it were a word) is a method used by molecular biologists to follow a particular sequence of DNA as it is passed on to other cells. RFLPs can be used in many different settings to accomplish different objectives. RFLPs can be used in paternity cases or criminal cases to determine the source of a DNA sample. RFLPs can be used determine the disease status of an individual. http://www.bio.davidson.edu/COURSES/genomics/method/RFLP.html
The use of RFLPs are genetic fingerprinting is based on?
Originally, yes. RFLP stands for Restriction Fragment Length Polymorphism. An enzyme which cuts DNA is added to a sample of DNA purified from blood or whatever tissue is available for the test. The enzyme will cut DNA only at particular sites, yielding many segments of cut DNA. The pattern of cuts can be seen by taking this partially digested DNA and running it through a gel to separate the smaller fragments from the larger fragments. This produces a set of distinctive bands that are essentially unique to every individual on the planet, although members of the same family may have similar patterns.For more details, refer to http:/en.wikipedia.org/wiki/DNA_profiling .
What are enzymes cutting DNA at specific sites to form restriction fragments called?
Enzymes that cut DNA at specific sites to form restriction fragments are called restriction endonucleases or restriction enzymes. These enzymes recognize specific DNA sequences and cleave the DNA at or near these sequences, generating DNA fragments with defined ends.
How are RFLP made?
A DNA sample is broken into pieces by restriction enzymes and the resulting fragments are separated according to their lengths by gel electrophoresis. RFLP analysis was the first DNA profiling technique inexpensive enough to see widespread application. But isn't as widely used now.
What tools of recombinant DNA technology is incorrectly paired with its use?
DNA polymerase-polymerase chain reaction to amplify sections of DNA reverse transcriptase-production of cDNA from mRNA DNA ligase-cutting DNA, creating sticky ends of restriction fragments restriction enzyme-analysis of RFLPs electrophoresis-separation of DNA fragments
What is a person's unique RFLP pattern?
sometimes, there isn't enough DNA found at a crime scene to be properly analyzed by forensic scientists. RFLPs are used to make exact copies of the DNA sequence so that it can be better analyzed in the lab.
