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Pasteur proved the germ theory at the same time disproving the theory of spontaneous generation. Pasteur's research showed that the growth of microorganisms was responsible for spoiling beverages, such as beer, wine and milk. Beverage contamination led Pasteur to the idea that microorganisms infecting animals and humans cause disease. He invented artificially weakened bacteria for anthrax vaccinations in cattle and invented rabies vaccine.

Because of his study in germs, Pasteur encouraged doctors to sanitize their hands and equipment before surgery. Prior to this, few doctors or their assistants practiced the procedure of washing their hands and equipment.

Vaccines and antiseptics brought great advances in medicine, leading to higher survival rates and extended life expectancy.

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