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If you do open one or more of them, you can contaminate them with microbes. These will most likely not be the ones you are trying to culture. It can give you a false negative.

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10y ago

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Why is it important not to open sterile plates?

If you do open one or more of them, you can contaminate them with microbes. These will most likely not be the ones you are trying to culture. It can give you a false negative.


What is used to grow colonies of bacteria away from open air contaminants?

Agar plates are commonly used to grow colonies of bacteria away from open air contaminants. Agar is a gel-like substance that provides nutrients and a solid surface for bacterial growth while protecting the colonies from airborne contaminants. By streaking the bacteria onto the agar plate, researchers can isolate and study pure colonies of bacteria.


What way should a sterile pack open?

A sterile pack should open away from the body to prevent contamination. This technique helps maintain the sterility of the contents by minimizing the risk of touching the sterile field or the items inside. It's important to handle the pack carefully and ensure that the outer surface, which is considered non-sterile, does not come into contact with sterile areas or instruments. Always follow specific protocols and guidelines relevant to the procedure being performed.


What is the difference between sterile and non-sterile gauze?

One is guaranteed to have no microorganisms on it inside the sterile packaging, the other is clean but is not guaranteed to have no microorganisms on it.


What has the author Pam Agar written?

Pam Agar has written: 'End to end' -- subject(s): Recreational use, Parks, Open spaces, History


Once a sterile tray is opened how long is it sterile?

As soon as you open a dry sterile container it is not sterile any more due to stuff falling on it. Studies do show that the longer a sterile field is exposed the higher rate of contamination. There is less contamination with a covered field. Jim RN


Why did you have to incubate the agar plates which were never opened during the ubiquitous microbes exercise?

They're presumably the controls. You need to treat them exactly the same as the others, except for one specific thing. In this case, it's that they were never opened. Otherwise, you wouldn't be able to know for certain whether the difference in the results was because you didn't open them or because you didn't incubate them.


Does makeup concealer infect open wounds?

Makeup concealer is not produced or marketed as a sterile compound. As such, there is a distinct possibility of infecting an open wound by rubbing a non-sterile cream into it. Especially with facial wounds, but actually with all wounds, until they heal, you should minimize exposure to non-sterile adulterants.


How can streaked plates become contaminated?

If it is left open on the bench it can be contaminated by bacteria in tyhe air, e.g. micrococci. If the loop was not flamed adequately before innoculation this can also cause contamination. and also if the medium used was not sterile in the first place the plate will be contaminated.


Should a bandage be applied with sterile technique?

If the bandage comes in contact with an open wound, then yes it should. If a bandage is securing a sterile dressing that completely covers the wound, then as the sterile field is already intact, simple prophylacsis will do.


What is the difference between sterile and non-sterile dressings?

Sterile dressings are free from microorganisms, making them suitable for covering wounds to prevent infection. Non-sterile dressings may have some microorganisms present and are typically used for minor injuries or to provide cushioning. Sterile dressings are essential for sterile procedures and when dealing with open wounds to reduce the risk of infection.


What happens if we transfer the culture from an open broth tube into a sterile broth tube?

Transferring culture from an open broth tube to a sterile broth tube introduces microorganisms from the original culture into the sterile environment. If the transfer is done aseptically, the sterile broth will facilitate the growth of the introduced microorganisms, leading to an increase in their population. However, any contamination from the open tube could also introduce unwanted microbes, potentially affecting the purity and results of the culture. Proper aseptic techniques are crucial to minimize contamination during this process.