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We use non absorbent cotton for plugging of test tubes or other glassware. If we would use absorbent cotton then during sterilization (autoclaving) sterile conditions will not be maintained as cotton will absorb water and microbes will swim from outside to inside. However, non absorbent cotton does not get wet and chances of contamination are decreased significantly.

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Q: Why is non-absorbent cotton preferred for plugging of test tubes?
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