due to virulence plasmid loss during subculturing
Aseptic techniques are essential for sub-culturing to prevent contamination from unwanted microorganisms, which can compromise the results of experiments or cultures. By maintaining a sterile environment, researchers ensure that only the intended microorganisms grow, allowing for accurate observation and analysis. These techniques also protect the integrity of the samples and minimize the risk of introducing pathogens or spoilage organisms. Overall, aseptic methods are crucial for maintaining the reliability and validity of microbiological work.
Because in culture outside of the host organism microbe or virus are not subject to immune response pressure, so their own genes that are responsible for virulence become not necessary and can be lost due to lack of selective pressure. Also, composition of the culture media can be such, that some important genes that are needed to pathogen's survival in its normal host stop being necessary. The whole thing is a lot like an atrophy of muscles when they are not used.
That is apiculture
They are not individual organisms.
Apiculture or beekeeping.
No. For some reason no one had done eng-sub for the second series
Something done in secret is done underground, stealthily, surruptitiously, clandestinely, confidentially, covertly, slyly, furtively, sub rosa, sub judice, privately, in confidence, intimately, insidiously, obscurely or privily.
BY ripping it apart
tissue culturing
The past participle is cultured. The present participle is culturing.
Some cells only grow in culture.