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Mass balance in chromatography refers to the principle that the total mass of a substance entering a system must equal the total mass leaving the system, accounting for any accumulation within the system. This concept is crucial for understanding the distribution of analytes between the stationary and mobile phases during the separation process. It ensures that the quantity of material is conserved and helps in optimizing the efficiency and resolution of the chromatographic method. By applying mass balance equations, chemists can analyze and predict the behavior of compounds throughout the chromatography process.

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