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Neighbor-joining produces bifurcating nodes, which means that each internal node in the tree represents exactly two descendant lineages. This is because the algorithm reconstructs phylogenetic trees based on pairwise distances, consistently merging the closest pairs of taxa, resulting in a branching structure that splits into two at each node. Multifurcating nodes, which would indicate more than two lineages diverging simultaneously, are not generated by this method, as it focuses on a stepwise approach to build the tree.

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1mo ago

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