Stain with basic dyes cytoplasm shows blue precipitates
This description is characteristic of a reticulocyte, which is an immature red blood cell. The cytoplasmic network staining with brilliant cresyl blue represents residual ribosomal RNA in reticulocytes, helping assess bone marrow function and erythropoiesis. Reticulocytes can be useful in monitoring response to treatment for anemia or other conditions affecting red blood cell production.
By definition, a supravital stain is used to demonstrate the in vivo vital processes of cells that have been removed from the body. Supravital stains (New Methylene Blue N; Brilliant Cresyl Blue) are used in particular to stain reticulocytes for a manual retic count. Reticulocytes are immature RBC's released early from the bone marrow in response to anemia. Using Brilliant Cresyl Blue, the reticulocytes will show a "reticular" (mesh-like) network of residual rRNA that has not yet been extruded from the cell. Any RBC can be seen on a supravital stain, supravital stains are used to identify Heinz bodies in cases of G6PD deficiency anemia
Lavender or purple-top tubes are commonly used for reticulocyte counts, as these tubes contain the anticoagulant EDTA which helps preserve cell integrity for accurate analysis.
Why reticulocyte is increase in haemolytic anaemia?
methelyn blue
Proerythroblast, erythroblast, normoblast, reticulocyte
C21H21O4P
An immature red blood cell containing a cytoplasmic network that stains with brilliant blue is called a reticulocyte. Reticulocytes are typically found in the bloodstream following the release of young red blood cells from the bone marrow. They indicate active erythropoiesis or red blood cell production.
False
The counter or secondary stain used in the acid-fast stain technique is methylene blue.
A reticulocyte count is a blood test performed to assess the body's production of immature red blood cells (reticulocytes).
Yes.