Shaking a blood-filled tube vigorously can cause hemolysis, which is the rupture of red blood cells leading to the release of hemoglobin into the surrounding fluid. This can affect blood test results by altering the concentrations of certain analytes. It is recommended to gently invert blood tubes to mix contents without causing hemolysis.
White blood cells are typically found in the buffy coat layer of the hematocrit tube after centrifugation. This layer is located between the plasma and red blood cells and contains a mixture of white blood cells and platelets.
Blood may clot in a tube immediately after a blood test if there was insufficient mixing of the blood with the anticoagulant in the tube, causing the blood to coagulate. It could also happen if the blood was drawn too forcefully, causing damage to the blood cells and triggering the clotting process. Additionally, certain medical conditions or medications can increase the risk of blood clotting.
The FSC (Full Blood Count) test typically uses a lavender/purple top tube, which contains EDTA as an anticoagulant. This tube is used to collect the blood sample for analysis of various blood cell parameters like red blood cells, white blood cells, and platelets.
An SST (serum separator tube) contains a gel barrier that separates blood cells from serum when the tube is centrifuged. The gel allows for easier extraction of serum for laboratory testing without contaminating it with blood cells.
Red blood cells, serum, plasma
A Wintrobe tube is a laboratory device used for measuring the sedimentation rate of red blood cells in a sample of blood. It is a long, thin tube with calibrated markings that allow for the measurement of how quickly the red blood cells settle, which can provide information about inflammation and certain medical conditions.
For titers, blood can be collected in a red-top tube (serum separator tube) or a gold-top tube (serum separator tube with gel). The serum is then separated from the blood cells by centrifugation and used for the titer test.
Platelets and white blood cells
Nope - they're typically a flat disc.
The pink color at the top of a tube of blood collected in a FTM tube is due to a stabilizing gel present in the tube. The gel helps separate the serum or plasma from the blood cells. The lack of color in the middle and bottom of the tube is because the blood cells settle to the bottom due to their higher density, leaving the serum or plasma above them.
The Buffy coat in the hematocrit tube is composed of a thin layer of white blood cells and platelets. These components separate from the red blood cells due to differences in density during the centrifugation process. The Buffy coat is important for identifying and analyzing abnormalities in the white blood cells and platelets, such as infections or clotting disorders.