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the hemoglobin contained in a

known quantity of blood is converted into acid heematin by means of hydrochloric

acid. The colour is then compared with a standard tube containing acid haematin

of known strength" (Whitby and Britton, I935). The technique is straightforward

and can be learned in a few minutes. Despite the simplicity of the method, however,

there are numerous sources of error traceable not only to faulty technique but

also to unsatisfactory apparatus. The existence of fallacies became apparent while

making upwards of five hundred estimations of the Hb in connection with a clinical

investigation. The purpose of this article is to make a plea for standardization of

both technique and apparatus, and to try to estimate the importance of the various

sources of error that are encountered. For reference and discussion the standard

method taught to students in this hospital is set out below.

Clean Tube. Brush if necessary.

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