The purpose of creating a standard graph for hemoglobin determination is to establish a reliable reference that correlates the absorbance readings from a spectrophotometer to known concentrations of hemoglobin. This standard curve allows for accurate quantification of hemoglobin levels in patient samples by comparing their absorbance values to the established curve. It ensures consistency, precision, and reproducibility in hemoglobin measurements across different analyses. Additionally, it helps to identify any deviations or anomalies in hemoglobin levels, aiding in clinical diagnosis and monitoring.
Hemoglobin is broken down by recycling its Fe2+/3+ ions and then breaking it open, forming bilirubin. This is conjugated in the liver to prepare it for excretion.
Get the blocks out.
It is a method of hemoglobin estimation in blood. The process involves absorbing a drop of blood on a standard paper and comparing it with a standard specimen.
Drabkin's Solution is a mixture of potassium cyanide and potassium ferricyanide. What this solution does is hemolysis red blood cells and then hemoglobin is released.
cause
The minimum purity of a substance used to prepare a primary standard should be at least 99.8% to ensure accuracy and reliability of the standard solution. Impurities in the substance can lead to errors in the final concentration calculations.
1)keep the syllabus copy... 2) prepare standard text books with gate notes...
tallquist method
you get the right stuff
hemoglobin
To prepare the standard curve you will need linear graph paper, semi-log graph paper and absorbance. You can define your standard curve by finding the absorption or percent plot on the Y axis.