A 7-day-old culture may appear gram negative, when the organmisms are actually gram positive. Thus, S. epi would appear gram negative, and E. coli gram negative.
gram or wright stained smear, immunofluorescent stain and fatty acid, gaschromatography, cultures
When a thick smear is stained and viewed under a microscope, individual cells will be harder to distinguish due to overlapping. This can make it challenging to see details within individual cells, such as the internal structures or boundaries. It is typically recommended to use a thin smear for better visualization and interpretation of cellular details.
Candida species in a pap smear can be identified by examining the stained slide for characteristic yeast cells, which may appear as round or oval structures. These cells can often be seen in clusters or as single organisms, sometimes with a halo effect. Additionally, the presence of pseudohyphae may also indicate a Candida infection. Confirmatory testing, such as culture or molecular methods, may be used for definitive identification.
The medical term meaning stained blood smear is examined to determine the shape of individual red blood cells is called a peripheral blood smear. This test is commonly used to assess the morphology and appearance of red blood cells, which can provide valuable information about various blood disorders.
Covering the smear with bibulous paper during the endospore stain process helps to wick away excess stain and prevent the slide from drying out. This ensures that the endospores are properly stained and the background is clear for observation under the microscope.
Overheating the bacterial smear can result in distortion or destruction of the bacterial cells, making it difficult to observe them under the microscope. This can lead to inaccurate or inconclusive results when trying to identify the bacteria present on the smear.
sBoth thick and thin blood smears are taken from the finger tip of earlobe of malaria patient. This smear is kept in water to dissolve and remove the haemoglobin from the slide. The slide is stained and visualised under oil immersion lens to see the malaria parasites directly.
In a plaque smear wet mount of a direct stained slide, you would observe bacteria that are colored, allowing for easier visualization of their shapes and arrangements, such as cocci or bacilli. In contrast, an indirectly stained slide would show the bacteria as transparent against a colored background, highlighting their morphology without staining them directly. The indirect method often uses a counterstain to enhance contrast, making it easier to identify cellular structures. Both methods provide valuable insights into microbial presence and characteristics but emphasize different aspects of the bacteria.
A microscopic observation of a stained, peripheral blood smear (can maybe be used in evaluating blood disorders). http://www.brooksidepress.org/Products/OperationalMedicine/DATA/operationalmed/Lab/PeripheralSmear.htm
White blood cells can be observed in a light microscope by preparing a blood smear, staining the cells with a dye like Wright's stain or Giemsa, and then viewing them under high magnification. The cells will appear as small, irregularly shaped cells with a dark-stained nucleus and a lighter-stained cytoplasm.
No. You might be able to get cultures for a Pap smear, but seeing and feeling are part of the exam as well and that is not something you can do for yourself.
Cell fragility causes smudge cells to appear on peripheral smears. Addition of albumin prior to slide preparation essentially coats the fragile cells, thus keeping them intact on smear preparation.