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E. coli is isolated from food samples using a combination of selective enrichment and culturing techniques. First, the food sample is mixed with a selective enrichment broth, such as buffered peptone water, and incubated to allow any E. coli present to multiply. Next, samples from the enrichment broth are plated onto selective agar media, like MacConkey agar, where E. coli colonies can be distinguished by their characteristic color. Finally, suspected colonies are further confirmed through biochemical tests or molecular methods.

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2w ago

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