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FDA guidelines allow a maximum of 10.5 ml/kg body weight to be collected at each whole blood collection, including samples for mandated screening tests. The majority of blood centers use 500 ml collection bags (+ up to 50 ml for testing). The is approximately 12% circulating blood volume in a 50 kg (110 lb) donor, representing the lowest weight a blood donor may be. This volume was selected because studies have indicated a loss of 15% or more of circulating blood volume is associated with an increase in donor reactions, such as syncope, nausea and seizure activity.

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