Bovine Serum Albumin (BSA) is used in cell culture media. Animal cells require serum proteins in order to grow outside the body.
If we want to purify a specific protein from these cultured cells, the large amount of BSA present in the media poses a major problem in terms of contamination.
This is why BSA has to be separated from the cultured cells before beginning the protein separation process.
Antigen-antibody complexes would form a white precipitate between the bovine serum albumin and the swine serum albumin.
Bovine serum albumin is used as a standard in laboratory experiments because it is a stable protein that can help maintain the proper conditions for reactions to occur consistently and reliably.
The MW of BSA (bovine serum albumin) is approx 66 kilodaltons, or 66,000 grams per mole. Hope this helps! For future reference: 1kD = 1000g/mol.
BSA solution, or Bovine Serum Albumin solution, is a commonly used protein solution derived from cow serum. It is often used in biological research and diagnostic assays to block non-specific binding sites, stabilize proteins, or dilute samples. BSA solution is helpful in reducing background noise and enhancing specific interactions in experiments.
Bovine serum albumin (BSA) has an isoelectric point (pI) of approximately 4.7, meaning its overall charge is neutral at this pH. However, in practical applications, BSA is often used in solutions with a pH range of 6.5 to 7.5, where it remains soluble and functional. The exact pH can vary depending on the specific application or buffer used.
Another name for albumin is serum albumin.
measured serum calcium + (40-serum albumin)/50
A.T. Chow
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The purpose of a serum separator in a blood collection tube is to promote the separation of blood components. It contains a gel that forms a barrier between the serum and the blood cells when the tube is centrifuged. This allows for easy isolation and extraction of the serum for testing purposes.
New born calf serum is cheaper than FBS
serum,tiger tube