Ames test is used to test whether a certain substance is mutagenic. Liver extract is optionally added to simulate the effect of metabolism, as some compounds, like benzo[a]pyrene, are not mutagenic themselves but their metabolic products are.
The Ames test identifies chemical mutagens.
Mutants are not used, you have a culture of histidine dependent bacteria. This means that if you put them on a place with liver extract for food they should not make placks because there is no histidine pressent then you add a compound that you want to test if its a mutagen and if it is, you will start to see plaques form because the bacteria have mutated to becomes histadinde independent.
not with ames but with animes
The substances that are test positive with Ames test may or may not be carcinogenic for humans. Similarly, some substances that cause cancer in laboratory animals do not give a positive result Ames test. It is not possible to decide the carcinogenicity of any substances only depending on the Ames test.
The alanine aminotransferase test (ALT) can reveal liver damage. It is probably the most specific test for liver damage. However, the severity of the liver damage is not necessarily shown by the ALT test.
Ames test would typically be carried out in the field of biology, more specifically toxicology. The Ames test involves testing genetics, which involves taking a sample and examining it closely in a laboratory.
The test used for detecting chemicals with carcinogenic potential is the Ames test. This test uses bacteria to detect mutations induced by the chemical being tested, which can indicate its carcinogenic potential.
The TIBC test measures the amount of iron that the blood would carry if the transferrin were fully saturated. Since transferrin is produced by the liver, the TIBC can be used to monitor liver function and nutrition.
A bilirubin test is used to measure the levels of bilirubin in the blood, which helps in diagnosing conditions related to liver function and red blood cell breakdown. High levels of bilirubin can indicate issues such as liver disease, hemolytic anemia, or blockage of bile ducts.
No
infection of liver
The test compound is not necessarily the ultimate carcinogen. In many cases the resulting products in metabolism rather than the compound itself causes cancer. Say, you feed a lab animal with a test compound and it gets cancer. You analyze the animal and you will find that the initial compound is metabolized to other chemicals that directly caused cancer. So this is why a test compound need to be metabolically activated, that is, it has to be metabolized by body enzymes (rat liver has a lot!) and then added to Salmonella to see the mutagenicity. Clear?