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When pipetting, it's important to use the correct pipette for the volume being measured to ensure accuracy. Always pre-wet the tip with the liquid to be pipetted to improve precision and minimize sample loss. Additionally, maintain a consistent angle and depth while immersing the tip in the liquid, and avoid touching the tip to any surfaces to prevent contamination. Finally, use a slow, controlled technique when releasing the liquid to ensure complete transfer.
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How can one ensure they are properly pipetting?
To ensure proper pipetting, one should carefully read and follow the pipetting instructions, use the correct pipette size for the volume needed, hold the pipette vertically while dispensing, and always double-check the volume settings before pipetting. Additionally, it is important to practice good pipetting technique, such as releasing the plunger slowly and smoothly to avoid introducing air bubbles.
Is it safe to perform pipetting by mouth in a laboratory setting?
No, it is not safe to perform pipetting by mouth in a laboratory setting due to the risk of ingesting harmful chemicals or pathogens. It is recommended to use proper pipetting equipment to ensure safety.
