Sputum from a healthy person would have no growth on culture. A mixture of microorganisms, however, normally found in a person's mouth and saliva often contaminate the culture. they may be reported as normal flora contamination.
It can take anywhere from one to eight weeks to get Tuberculosis Sputum culture results. The test will determine if you have Tuberculosis.
The presence of bacteria and white blood cells on the Gram stain and the isolation of a microorganism from culture, other than normal flora contamination, is evidence of a lower respiratory tract infection.
It typically takes about 2-3 days for a sputum culture to show positive results. However, the exact time can vary depending on the specific laboratory testing procedures and workload.
Bacterial culture. Fungal culture. Viral culture
Sputum is material coughed up from the lungs and expectorated (spit out) through the mouth. A sputum culture is done to find and identify the microorganism causing an infection of the lower respiratory tract such as pneumonia.
Antibiotics in the person's system may prevent microorganisms present in the sputum from growing in culture.
It typically takes 1-2 days for preliminary results to be available, but final results may take 2-3 weeks as the bacteria need time to grow in the culture. The exact timeframe can vary depending on the specific laboratory and workload.
Spores are formed when cells are under unfavourable conditions, as for the bacteria they are means of survival. So the older the culture the higher the cell number in that culture, which means less nutrients for the cells. Under this conditions cells will start spore production. Depending on the "age" of the culture you can get a mixture of vegetative cells with spores inside and spores that are already released or mostly spores with rare vegetative cells, which means the cells are dead.
Sounds pretty typical.
What can cause germination of a disk
The specimen for culture should be collected before antibiotics are begun. The patient should first rinse his or her mouth with water. If tuberculosis is suspected, collection of sputum should be carried out in an isolation room.
Many types of microorganisms are normally found on a person's skin. Presence of these microorganisms is noted on a skin culture report as "normal flora."