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One notable bacterial infection that is difficult to culture is caused by Mycobacterium leprae, the causative agent of leprosy. This organism has a slow growth rate and cannot be cultured in vitro using standard laboratory techniques. Instead, it is typically studied using animal models, such as the footpads of mice or the armadillo, which can support its growth. This difficulty in culturing M. leprae complicates diagnosis and research on the disease.

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