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Non-lactose fermenting coliforms in urine are typically caused by the presence of certain bacteria, such as Enterobacter or Citrobacter species, which can be part of the normal flora or introduced through urinary tract infections (UTIs). These bacteria do not ferment lactose, which distinguishes them from typical E. coli strains that do. Factors contributing to their presence can include urinary stasis, catheter use, or compromised immune systems. Identifying these organisms is important for appropriate treatment and management of infections.
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Why is MacConkey agar inoculated with Urine specimen?
MacConkey agar medium differentiates between the lactose fermenting and nonlactose fermenting bacteria. Most of the bacterial commensals from our large intestine are lactose fermenting, Escherichia coli being one of them. When bacterial growths and colonies on MacConkey agar is pink coloured, then the organism is fermenting lactose from the medium, on the other hand, if transparent/transluscent, colourless growths and colonies appear on the medium, the bacterium does not ferment lactose. The flushing action of urine, make it sterile in an uninfected healthy person. In an event where the patient is suspected to have urinary tract infection (UTI), urine of patient is inoculated on MacConkey agar medium and incubated at 37 degree centigrade overnight. In most of the cases, UTI is caused by Escherichia coli that is lactose fermenting and produces pink coloured colonies on MacConkey agar medium. Although the urine is sterile, it may get contaminated by the organisms of skin microflora or by surrounding microbes due to improper collection of urine. In such cases, UTI is diagnosed by counting bacteria in urine sample. For significant bacteriuria (bacteria in urine), the count must be equal to or more than 1 lack bacteria per mL of urine. Answered by: M. F. Khan, Dept. of Microbiology, GMC, jagdalpur (CG)
What causes no urine?
Dehydration
What causes loss of glucose in the urine?
Insulin affects the concentration of glucose in the urine.
MacConkey agar inoculated with the urine specimen?
Most of the bacterial commensalism from our large intestine is lactose fermenting, Escherichia coli being one of them. When bacterial growths and colonies on MacConkey agar is pink coloured, then the organism is fermenting lactose from the medium, on the other hand, if transparent, colourless growths and colonies appear on the medium, the bacterium does not ferment lactose.The flushing action of urine, make it sterile in an uninfected healthy person. In an event where the patient is suspected to have urinary tract infection (UTI), urine of patient is inoculated on MacConkey agar medium and incubated at 37 degree centigrade overnight. In most of the cases, UTI is caused by Escherichia coli that is lactose fermenting and produces pink coloured colonies on MacConkey agar medium. Although the urine is sterile, it may get contaminated by the organisms of skin microflora or by surrounding microbes due to improper collection of urine.
What causes blood in the urine?
The most usual cause of blood in the urine is a kidney stone.
What causes orange urine in guinea pigs?
The chemicals in the urine
What causes dilute urine?
Matt dobbins
What causes pink urine in a female?
Aids
What causes pink urine?
Any number of causes. Food and the start of a period.
What hormone causes loss of glucose in urine?
insulin
What causes alcohol to produce more urine?
alchohol
What causes excess glucose in urine but not diabetic?
pyridium
