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No, chlamydia is spread by sex, not by toilet seats. STDs and STIs are called that for a reason: transmission usually only occurs through sexual/personal contact, or by bodily fluids such as seminal or vaginal fluids coming in contact with the mucous membranes of another person.

This makes it next to impossible to catch Chlamydia from sitting on a toilet because even if fluids were left by an infected individual, the fluids would only come in contact with the skin. Chlamydia lives for just a handful of minutes outside the body. Also, it can infect only the mucous membranes, which aren't in contact with a toilet seat during normal use.

If that's not convincing enough, consider the statistical evidence: why would half of all chlamydia cases occur in 15-26 year olds if you could get it from a toilet seat, given that toilet seat use is common in all age groups?
Chlamydia doesn't transfer via toilet seats.
No it can't be transferred from a toilet.

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