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During skeletal muscle contraction calcium ions expose myosin binding sites on to the actin. When a nerve sends a signal to a muscle to do some "action" the release of acetylcholine at the neuromuscular junction triggers calcium to be released into the cytoplasm. At rest, the cell has a low concentration of calcium. When the calcium concentration rises, calcium diffuses over to the muscle protein fibers and causes a conformational change. Calcium binds to the troponin and causes it to rotate slightly which forms a crossbridge that drags along the actin fiber and shortens it thus creating muscle fiber contraction.

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