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What is copro antigen and what is its role in detection of parasites?
antigen prepared from faecal sample of infected animal
What are the application of radial immunodiffusion?
1.used for determining the antigen concentration of unknown sample. 2.to identify the specific viral antigen from the mixed viral sample.
What are the applications of radial immuno diffusion?
1.used for determining the antigen concentration of unknown sample. 2.to identify the specific viral antigen from the mixed viral sample.
Hemagglutination-inhibition test description?
It is the measures of the ability of soluble antigen to inhibit the agglutination of antigen-coated red blood cells by antibodies. In this test, a fixed amount of antibodies to the antigen in question is mixed with a fixed amount of red blood cells coated with the antigen (research on passive hemagglutination). Also included in the mixture are different amounts of the sample to be analyzed for the presence of the antigen. If the sample contains the antigen, the soluble antigen will compete with the antigen coated on the red blood cells for binding to the antibodies, thereby inhibiting the agglutination of the red blood cells.
What color of tube is used on a psa?
The tube color for a prostate-specific antigen (PSA) test is typically lavender. This color is used to indicate that the tube contains an additive that helps stabilize the blood sample for accurate testing.
What do you mean by qualitative elisa and quantitative elisa?
Qualitative ELISA determines the presence or absence of a specific antigen or antibody in a sample. It provides a yes/no answer. Quantitative ELISA measures the amount of antigen or antibody present in a sample, providing a numerical value to indicate the concentration of the analyte.
In indirect elisa the enzyme liked antibody will attach to?
In an indirect ELISA, the enzyme-linked antibody attaches to the target antigen that has been immobilized on the microplate. This allows for the detection of the antigen through the enzyme's activity, which produces a signal that indicates the presence of the target antigen in the sample.
What happens to the sample mean as the sample size increases?
With a good sample, the sample mean gets closer to the population mean.
What does immunoprecipitation measure?
the quantity of precipitate, which forms after the reagent antibody (precipitin) has incubated with the sample and reacted with its respective antigen to form an insoluble aggregate.
What happens to the distribution of the sample means if the sample size is increased?
the means does not change
What reason could there be for a negative result when the antigen is actually present?
A negative result despite the presence of the antigen can occur due to several factors, including improper sample collection or handling, insufficient sensitivity of the test used, or the timing of the test in relation to the infection stage. Additionally, the presence of interfering substances in the sample can hinder the test's ability to detect the antigen. Laboratory errors, such as incorrect test procedures or equipment malfunctions, might also contribute to false-negative results.
What is high dose hook effect?
High Dose Hook Effect refers to measured levels of antigen displaying a significantly lower absorbance than the actual level present in a sample. This appears when a simultaneous ELISA assay is saturated by a very high concentration of sample antigen binding to all available sites on both the solid phase antibody as well as the detection antibody and thereby preventing the sandwich-formation. The antigen-saturated detection antibodies in solution will be washed off giving a falsely low signal. A "hook" is observed in the curve when data is plotted as a signal versus antigen concentration.
