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How is the antinuclear antibody test prepared?
The antinuclear antibody test is done by adding a person's serum to commercial cells mounted on a microscope slide. If antinuclear antibodies are in the serum, they bind to the nuclei of cells on the slide.
What is added to the serum in the antinuclear antibody test?
a second antibody is added to the mixture. This antibody is "tagged" with a fluorescent dye so that it can be seen. The second antibody attaches to any antibodies and cells bound together
What is a Wasserman test?
It is a diagnostic test for syphilis that checks the blood serum reaction to an antibody.
Why you use serum of animal against which your secondary antibody is raised for blocking the membrane?
It prevents non-specific binding of the secondary antibody, and thus reduced background.
What are the types of susceptibility test for antibiotics?
Kirby-Bauer method (uses disk diffusion method)E-test (uses capillary disks)Spiral Gradient Endpoint test (results are interpreted by computer)Serum Bactericidal test (test amount of antibody present in patient's serum)
What is a serology lab?
Serology, the study of serum, is the science that studies antigen-antibody or immunologic reaction of the body,using a serum specimen. A primary role of the serological lab is to diagnose infectious diseases by observing the presence of an immune antibody in the pt.which resulted from inf. or entry of the pathogen (antigen) into the body.
When would the antibody titer in a host be the highest a.during the period of illness b.in the acute phase c.in a healthy person recently exposed to a disease d.in the convalescent serum?
convalescent serum
Can allergy shots cause serum sickness?
No. Even though the treatment solution for allergy shot is sometimes called "allergy serum", there is no serum in these solutions. Serum sickness occurs when a large dose of an animal serum protein or a large dose of some medications are given and the immune system make high doses of immune antibody against that protein. Small capillaries in the joints, kidneys, skin, etc get plugged up by the clumps formed when the antibody joins with the protein. Allergy shots use very week doses because the person is already allergic to the treatment solution. Large doses are required to develop serum sickness.
How is the amount of antimyocardial antibodies measured?
serum is diluted, or titered, and the test is done again. The serum is then further diluted and the test repeated until the serum is so dilute that fluorescence is no longer seen. The last dilution that showed fluorescence is the titer reported.
What is serum insulin?
It means the amount of insulin found in the watery part of the blood called serum.
What term is used to describe a broad scope of laboratory tests that use specific antigen and serum antibody reactions?
The term used to describe a broad scope of laboratory tests that utilize specific antigen and serum antibody reactions is "immunoassay." These tests are designed to detect and quantify substances, such as proteins or hormones, in a sample by measuring the interaction between an antigen and its corresponding antibody. Immunoassays are widely used in clinical diagnostics, research, and various fields of medicine.
Explain briefly the contents of the Landsteiner's rule of reciprocity?
Landsteiner's rule states that when an antigen is on a red blood cell, the corresponding antibody is never present simultaneously. Instead, the reciprocal red cell antigen is present in the plasma or serum.(Ex. A type A individual has anti-B in their serum.)
