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During breathing, the primary muscles involved are the diaphragm and the intercostal muscles. The diaphragm contracts to create a vacuum that allows air to flow into the lungs, while the intercostal muscles assist by expanding and contracting the rib cage. During inhalation, these muscles work together to increase thoracic volume, and during exhalation, they relax to help expel air from the lungs. Additional accessory muscles may be recruited during heavy breathing or respiratory distress.

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