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Lupus is diagnosed using a combination of clinical evaluation and laboratory tests. Key tests include the antinuclear antibody (ANA) test, which detects autoantibodies that are commonly present in lupus patients. Other important tests may include anti-double-stranded DNA and anti-Smith antibodies, as well as blood tests to assess kidney function and inflammation markers. A thorough medical history and physical examination are also crucial in identifying characteristic symptoms of lupus.

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What is ANA in science?

ANA stands for antinuclear antibodies. These are autoantibodies produced by the immune system that mistakenly target and attack the body's own cell nuclei. ANA testing is often used to help diagnose autoimmune diseases such as lupus and rheumatoid arthritis.


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What color tube is used for lupus test?

The most common tube color used for lupus testing is a red-top tube, which contains no anticoagulant. This allows for the blood to clot before testing. Additionally, a lavender-top tube containing EDTA anticoagulant may also be used for specific lupus tests.


What blood test tube is used to test for lupus?

A variety of blood tests are used to help diagnose lupus but there is no single marker. Examination of blood cells (red, white, platelets) is usually performed. Lupus patients may be anemic, have low white counts, or be low on platelets. A metabolic panel will be done to rule out other diseases or conditions that have symptoms similar to lupus. Sedimentation rate, CRP and ferratin tests will reveal inflammation inside the body. FANA ( fluoresecnt antinuclear antibody), anti double stranded DNA. anti Smith, LE prep, and other autoantibody screenings may be performed. Tests for antiphospholipid antibody syndrome may also be performed. There is no definitive test. 10 million Americans have a positive ANA, but only about 1.5 million have lupus. Of the 1.5 million people with lupus 95-98% will have a negative ANA. The other tests present the same problems. If a person has a positive antiSmith then they do have lupus. But a large percentage of lupus patients do not have this antibody. All in all, there are 118 autoantibodies that may or may not be present.


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How is lupus diagnosed?

To have lupus a patient must have four out of eleven abnormalities from a list established by the American Rheumatism Association. These abnormalities are:1. Malar rash2. Discoid rash3. Photosensitivity4. Mucosal ulcers5. Serositis6. Arthritis7. Renal disorders8. Neurological disorder9. Haematological disorder10. Immunologic disorder11. Anti-Nuclear Antibody The American College of Rheumatology established these 11 criteria as a means of determining whether or not a patient qualifies to enter lupus clinical trials. In the process of diagnosing lupus, these are helpful but not absolute, some criteria carrying more weight than others. For example, a kidney biopsy that confirms lupus nephritis determines a diagnosis of lupus whether or not any of the other 11 criteria are present. A skin biopsy that confirms cutaneous lupus is all that is needed to make that diagnosis as well. Lupus is diagnosed based on these steps and information: # Medical history # Symptoms # Wide variety of lab tests # After other disorders have been ruled out It should be noted that 10 million Americans have a positive Antinuclear antibody titer (ANA) but only 1.5 million have lupus. Of that 1.5 million with lupus, 5% will have a negative ANA titer. This, according to Daniel Wallace, MD in The Lupus Book.


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