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Two or more precipitant bands can form in an antigen-antibody experiment due to the presence of multiple antigenic epitopes that react with different antibodies, leading to the formation of distinct immune complexes. Additionally, variations in antibody concentrations or affinities can result in the formation of different-sized complexes that precipitate at varying rates, creating multiple bands. This phenomenon may also occur if the sample contains multiple antigens that can bind to the same antibody, resulting in the formation of separate precipitate zones.

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