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Why are Mycobacterium digested with sodium hydroxede for 30min prior to staining?
First of all I suggest you to check and correct the mistakes which you have done in your question. It is sodium hydroxide (NaOH) rather than sodium hydroxede, the mycobacterium is not digested rather sodium hydroxide (NaOH) is used to homogenize and decontaminate the sputum sample which is generally collected in case of pulmonary tuberculosis. The sputum may contain the mycobacterium along with other normal commensal flora of the upper respiratory tract and the oral cavity. The load of mycobacterium may be less and it may be entrapped in the mucous part of the sputum sample, therefore, the process of homogenization is done using sodium hydroxide to digest the mucous, liberate the entrapped mycobacterium and destroy the contaminating commensal flora.
Homogenization and decontamination is generally done by different methods but Petroff's method is the most commonly used. The sample is then neutralized by adding HCl (hydrocholoric acid) and is used for smear and culture.
What else can I help you with?
How is sodium digested?
Sodium is not digested in the traditional sense like food. It is absorbed in the small intestine as an electrolyte during the digestive process. Once absorbed, sodium helps maintain the body's fluid balance and is crucial for nerve function and muscle contractions. Any excess sodium is eliminated by the kidneys through urine.
Does sodium monofluorophosphate stain teeth like stannous fluoride does?
No, sodium monofluorophosphate does not stain teeth like stannous fluoride. Staining of teeth is more commonly associated with stannous fluoride, whereas sodium monofluorophosphate is less likely to cause staining.
What is the function of sodium bicarbonate with Merthiolate in Gram stain?
Sodium bicarbonate is used to adjust the pH of the staining solution in the Gram stain procedure. Merthiolate is used as a mordant to enhance the crystal violet staining in the Gram stain. Together, they help differentiate between Gram-positive and Gram-negative bacteria based on their cell wall characteristics.
What is the technique used to detect corneal abrasion?
Corneal abrasions are usually detected using a slit-lamp with sodium-fluoroscein dye and blue light, which utilizes the concept of fluorescence to pinpoint corneal staining from a corneal abrasion.
How do you decolourise schiffs reagent?
To decolorize Schiff's reagent, you can add a reducing agent like sodium metabisulfite or sodium bisulfite dropwise until the color disappears. This process helps to reverse the original color change caused by the reagent reacting with aldehydes or other carbonyl compounds.
What is the name for NaClO4?
sodium chlorateNaClO3 = Sodium Chlorate
What is the name for the compound with the formula NaF?
Sodium fluoride is an ionic compound with the formula NaF.
What are the formula units for sodium sulfide sodium sulfite and sodium sulfate?
Sodium sulfide: Na2S Sodium sulfite: Na2SO3 Sodium sulfate: Na2SO4
What type of atom is in sodium chloride?
Sodium chloride has two atoms in the formula unit (NaCl): sodium and chlorine.
What is a word equation for sodium and chlorine?
Sodium + Chlorine ---> Sodium Chloride I think that is correct
What are the three common compounds formed for sodium?
sodium chloride, sodium carbonate, sodium bicarbonate
What is the chemical name of Na2S2O4?
Sodium thiosulfate or Sodium Hyposulphite.
