The API 20e has dry reagents in the tubes. Adding the sample adds the initial moisture, but it must be kept moist during incubation for the reactions to fully develop. Some samples may take up to 48 hours at 37 degrees C.
The purpose of water in the tray of an API 20E system is to create a humid environment that helps the bacteria's growth and metabolism during the biochemical testing process. This process is essential for accurate identification of the organism based on its biochemical reactions.
The country located at coordinates 25N, 20E is Egypt. It is situated in North Africa and bordered by the Mediterranean Sea to the north.
The country located at 55N and 20E coordinates is Finland.
The country located at approximately 60N and 20E is Finland.
The fourth digit must be equal to or greater than four because if the test for GLU is negative, the bacteria is not enterobacteria. This would defeat the purpose of the API 20E test. GLU has a number of four, so even if the GEL or VP test is negative, the fourth digit for that bracket of three tests will be at equal to four.
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Kraków
The oxidase test is performed to determine if the culture contains cytochrome c oxidase enzyme, which helps in distinguishing between oxidase-positive and oxidase-negative organisms. This information is important for selecting the appropriate biochemical tests in the API 20E and Enterotube II identification systems, as these tests are designed to work best with specific types of bacteria based on their oxidative characteristics.
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Russia
The coordinates 40N and 20E pass through Greece.
The coordinates 40N 20E pass through Albania. Tirana is the capital and most populous city of Albania a country in southeastern Europe.