There are several gunpowder contaminants such as the fertilizer, detergent, match head, even soil and cigarettes. They contain nitrates which gives similar result to gunpowder residues when treated with Diphenylamine (DPA) Reagent.However, their reaction differ from gunpowder nitrates from the characteristics of the specks as well as the distribution of the specks. Usually a gunpowder contaminants will show a smear of blue color .
Reagent Blank : Take reagent and add deionised water (in place of sample to be tested). Now measure the OD at specific wavelength --> this OD is your reagent blank. Substract this OD from your test result (with sample) to avoid any false +ve effect due to colour of reagents itself.Sample Blank : Take sample and measure the OD without adding reagents --> this OD is your sample blank. Substract this OD from your test result to avoid any false +ve effect due to colour and turbidity of sample itself. As it is the fact that colour and turbidity of each sample would vary from one to another.So now it is clear that Reagent blank is used to avoid bias due to colour of reagents and Sample blank is used to avoid bias due to sample itself.
The oxidase reagent needs to be fresh because it contains the enzyme cytochrome c oxidase, which can degrade over time, leading to false-negative results if it is not active. Using fresh reagent ensures the accuracy of the test results.
Returning excess material to the original container can introduce possible contamination and compromise the stock solution. It's best practice to avoid returning unused reagent to its original container to ensure accuracy and purity of the stock solution for future experiments.
False negative color reactions can occur due to several factors, including insufficient sample concentration, improper reagent handling or storage, and interference from contaminants or substances within the sample. Additionally, environmental conditions such as temperature and pH can affect the chemical reactions needed for color development. Lastly, expired or degraded reagents can also lead to inaccurate results.
Yes, an error in a pregnancy test could lead to a false positive or false negative result.
No, folliculitis will note cause a false positive HSV2 result.
False. It is impossible not to have touched anything.
False. It is impossible not to have touched anything.
False. It is impossible not to have touched anything.
Oxidase reagent needs to be fresh because it is sensitive to degradation and can lose its reactivity over time. When the reagent is old or improperly stored, it may not accurately detect the presence of cytochrome oxidase in microbial cultures, leading to false-negative results. Fresh reagents ensure reliable and consistent performance in biochemical tests.
The Chinese invented gunpowder in 850 B.C.
false