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Methicillin-resistant Staphylococcus aureus (MRSA) is difficult to detect by routine susceptibility testing because it often exhibits a heterogeneous population of bacteria, with some cells expressing resistance and others not. This variability can lead to false-negative results in standard tests, as the presence of resistant strains may not be evident in the overall culture. Additionally, MRSA can sometimes demonstrate altered expression of resistance genes, complicating the interpretation of susceptibility profiles. Therefore, specialized testing methods may be necessary to accurately identify MRSA in clinical samples.

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