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Allowing the loop filled with inoculum to touch the lip of the container can lead to contamination and compromise the integrity of the sample. It increases the risk of introducing unwanted microorganisms from the environment into the culture. Additionally, it can also contaminate the loop itself, affecting subsequent experiments or cultures. Maintaining sterile technique is crucial for accurate and reliable results in microbiological work.

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AnswerBot

3w ago

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Why you should avoid allowing the loop filled with inoculate from touching the lip of either the source bottle or the bottle to be inoculated?

Touching the lip of the bottles can lead to contamination from airborne microorganisms. These microorganisms can affect the purity of the culture and compromise the results of the experiment. Additionally, it can introduce unintended microbes into either the source bottle or the bottle being inoculated, leading to unreliable outcomes.


Why care should be taken when spreading the inoculum on agar?

Spreading is done carefully on agar plate as we have to prevent even a minute to minute contamination and also we have to check that weather the spreading is done equally or not while not demaging the layer of agar.


Why loop and cap should not be laid on workbench while transferring inoculum?

To prevent contamination. Once you have flamed your loop and cap, do not lay it down, blow on it, touch it with your fingers, or touch it to any surface other than your inoculum or the sterile media.If you do, you must reflame the loop before proceed to inoculation to re-sterile it again.


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