Staphylococcus aureus does not typically grow on MacConkey agar, as this medium is selective for Gram-negative bacteria, particularly Enterobacteriaceae, due to its bile salts and crystal violet dye that inhibit Gram-positive organisms. However, if S. aureus is observed on MacConkey agar, it may be due to contamination or the presence of certain strains that can tolerate the inhibitory conditions. Generally, for the isolation of S. aureus, media such as mannitol salt agar is more appropriate since it is selective for staphylococci.
yesTypically, MSA is used to isolate and differentiate various staphylcocci, some of which are pathogenic, like S. aureus, or non pathogenic like S. epidermidis. Staph are the only kind of bacteria that will grow on MSA. S. aureus is the only staph that ferments manitol. Meaning, MSA can be used to isolate pathogenic from non-pathogenic staph.source: biology major
Staphylococcus will not grow on Mac since crystal violet inhibits the growth of Gram positivesfmchinea: Actually some species of staphylococcus grow on MacConkey's plate including Staphylococcus aureus.
No, Alcaligenes faecalis will not grow on MacConkey agar. MacConkey agar is selective for gram-negative bacteria that ferment lactose. Alcaligenes faecalis is a gram-negative bacteria that does not ferment lactose, so it will not grow on MacConkey agar.
MacConkey agar is designed to grow Gram-negative bacteria and also contains crystal violet dye which inhibits the growth of Gram-positive bacteria. Since S. aureus is Gram-positive it should not grow on MacConkey agar. No. MacConkey agar inhibits MOST of the Gram-positive bacteria. There are some Gram-positive bacteria that can tolerate bile-salt are able to grow in MacConkey agar. Enterococcus and some species of Staphylococcus are able to grow in MacConkey. (lack citation though)
Eikenella corrodens generally does not grow on MacConkey agar because it is a fastidious organism that requires enriched media for growth. MacConkey agar is selective for gram-negative bacteria and primarily supports the growth of enteric bacteria, which Eikenella is not. Instead, Eikenella grows well on blood agar or other enriched media that provide the necessary nutrients.
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MacConkey agar
Lac+ bacteria is acid base color indicator that lowers the pH of the MacConkey agar. The MacConkey agar contains neutral red dye, lactose, peptone, and crystal violet dye.
MacConkey agar contains agar as a solidifying agent which solidifies at room temperature to provide a solid surface for bacterial growth and colony isolation. MacConkey broth, on the other hand, does not contain agar and remains liquid at room temperature to allow for easier culture of bacteria in liquid medium for metabolic studies or growth analyses.
Clostridium species do not grow on MacConkey agar because they are anaerobic bacteria that require an oxygen-free environment, while MacConkey agar is designed for the growth of aerobic and facultatively anaerobic Gram-negative bacteria. Additionally, MacConkey agar is selective for lactose fermenters, and Clostridium does not ferment lactose. Therefore, Clostridium cannot be cultured on this medium.
Enterococcus faecalis typically does not grow on MacConkey agar as this selective and differential medium is primarily used for isolating and differentiating members of the Enterobacteriaceae family, which are mostly gram-negative bacteria. Enterococcus species are gram-positive bacteria and do not ferment lactose, the key ingredient in MacConkey agar that allows for differentiation.
Most gram positive bacteria do not grow on MacConkey agar or Eosin Methylene Blue (EMB) agar, as these are selective media designed to inhibit the growth of gram positive organisms. So, typically, you would not expect a gram positive bacteria to grow on these media.