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WBC diluting fluid is designed to lyse red blood cells (RBCs) while preserving white blood cells (WBCs) for counting and analysis. The lysis occurs due to the specific osmotic properties and chemical composition of the diluent, which affects the RBC membranes but not the more robust WBC membranes. This selective lysis allows for accurate WBC enumeration without interference from RBCs. The fluid typically contains agents that disrupt the lipid bilayer of RBCs, leading to their breakdown while maintaining the integrity of WBCs.
What else can I help you with?
Why wbc diluting fluid hemolyze rbc?
The distilled water present in the WBC diluting fluid is not isotonic and therefore is responsible for the destruction of RBCs. DR. Umesh Ghate and Shruti Salian
What is the chemical composition of RBC diluting fluid?
RBC diluting fluid commonly consists of an isotonic saline solution containing electrolytes and buffers to maintain the stability of red blood cells during counting. The composition may vary based on the specific laboratory protocol or commercial product used.
Why bulb of wbc smaller than rbc pipette?
Rbc pipette is bigger than the wbc pipette to dilute the blood with fluid.
What is the Difference between rbc thoma pipette and wbc thoma pipette?
The difference between the two is that the RBC Diluting pipette counts the erythrocytes (Red blood cells) while the other one counts the leukocytes (White blood cells) and that the pipette (RBC) itself has a more circular body compared to WBC diluting pipete. On the other hand, WBC diluting pipette has a pointed tip unlike the RBC Diluting pipette. -Fer Philippines <3
How do you count the RBC and WBC counting?
counting of Rbc and Wbc is done throug hemocytometer .duilting fluid are needed to destory unwanted things which can confuse us in various ways .
What is the ratio of WBC to RBC?
1 to 2 WBC TO 1000 RBC
Role of RBC lysis solution in DNA isolation?
When isolating DNA from blood, white blood cells (WBC's) are the target. This is because RBC's do not contain a nucleus and therefore do not contain DNA. The function of the lysis buffer is to help in the lysis (or breaking) of white blood cells. WBC's must first be lysed so that the DNA may be released from inside the cell.
When a MCH is high and an RBC is low what can cause this?
When a MCH and an RBC and WBC is low, what can cause this"
Difference between rbc and wbc pipette?
RBC pipette has graduations up to mark 101 while WBC has graduations up to mark 11. The size of the bulb of RBC pipette is larger than the bulb of WBC Size of the lumen(the hollow structure)in RBC is smaller than that of the WBC pipette
What prediction can you make with rbc's and wbc's?
If rbc stands for red blood count and wbc stands for white blood count, then you may be able to predict if someone is becoming anemic.
Are wbc and rbc hgb hct counts low with cancer?
I believe that cancer does make your WBC lower.
How do you explain the cellular components of blood?
RBC,WBC,Plasma
