How do you reconstitute heparin in PBS?

Answer 1

To reconstitute heparin in phosphate-buffered saline (PBS), first determine the required concentration and volume for your application. Measure the appropriate amount of heparin powder, then add a calculated volume of sterile PBS to achieve the desired concentration. Gently mix the solution to ensure complete dissolution, avoiding vigorous agitation to prevent degradation of the heparin. Finally, filter the solution if necessary and store it at the recommended temperature.