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a suitable strain of corynebacterium diphtheriae is grown on a liquid medium and incubated at 37 C for 7-10 days. After period sufficient concentration of phenol is added to ensure sterilty then it is filtered through a bacteria proof filter. The strerile filtrate is diphtheria toxin with 0.4 percenet formaldehyde solution and incubated at 37 C for 3-4 weeks. The resulting product is diphtheria toxoid or formal toxoid.

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