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Queen Elizabeth's government proved the threat from Mary, Queen of Scots, through various plots and conspiracies that linked Mary to attempts to depose Elizabeth. The most significant was the Babington Plot in 1586, where Mary was implicated in a scheme to assassinate Elizabeth and place Mary on the English throne. This evidence, along with Mary's claim to the English crown and her Catholic support, fueled fears of a Catholic uprising in England. Ultimately, these threats led to Mary's trial and execution in 1587.

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