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The Minimal Inhibitory Concentration (MIC) of an antibiotic is determined through laboratory testing, typically using a broth dilution or agar diffusion method. In the broth dilution method, serial dilutions of the antibiotic are prepared in a growth medium, and a standardized inoculum of bacteria is added to each dilution. The MIC is the lowest concentration of the antibiotic that prevents visible growth of the bacteria after incubation. In the agar diffusion method, the antibiotic is applied to an agar plate inoculated with the bacteria, and the MIC is inferred from the size of the inhibition zone around the antibiotic disc.

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