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The region that caused significant decreases in Native American populations was primarily the eastern United States, particularly due to the impact of European colonization. Diseases introduced by Europeans, such as smallpox and influenza, decimated indigenous populations who had no immunity. Additionally, violent conflicts, forced removals, and the disruption of traditional ways of life further contributed to population declines. These factors combined led to dramatic reductions in Native American numbers and significant cultural upheaval.

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