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The biggest challenge faced by Jamestown colonists was a severe lack of food and clean water, exacerbated by harsh environmental conditions and poor planning. Many settlers were unprepared for the demands of survival in the New World and focused more on searching for gold than establishing sustainable agriculture. This led to starvation, disease, and high mortality rates, particularly during the "Starving Time" of 1609-1610, when many colonists perished. Additionally, conflicts with Indigenous peoples further complicated their struggle for survival.

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