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The population of Jamestown plummeted between 1609 and the spring of 1610 primarily due to a combination of severe famine, disease, and conflicts with Native Americans. The winter of 1609-1610, known as the "Starving Time," saw food supplies dwindle, leading to starvation among settlers. Additionally, the lack of proper sanitation and the spread of diseases contributed to the high mortality rate. By the spring of 1610, the settlement had lost a significant portion of its population, reducing it to just a few dozen survivors.

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