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Right hand and left hand threads differ in the direction they tighten or loosen. Right hand threads tighten when turned clockwise and loosen when turned counterclockwise, while left hand threads tighten when turned counterclockwise and loosen when turned clockwise. This difference affects the assembly process because using the wrong type of thread can lead to parts not properly securing or coming loose during operation. It is important to match the correct thread type to ensure a secure and stable assembly.

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