Enzymatic activity is primarily associated with proteins, which are organic molecules made up of amino acids. These proteins act as enzymes, catalyzing biochemical reactions by lowering activation energy. Some RNA molecules, known as ribozymes, can also exhibit enzymatic activity by catalyzing specific reactions, demonstrating that not only proteins but also certain nucleic acids can function as enzymes.
Ribozymes, which are RNA molecules with catalytic activity, are capable of catalyzing some types of enzymatic reactions. They can act as enzymes and facilitate various biochemical reactions in cells.
Exonuclease activity refers to the enzymatic function of removing nucleotide residues from the ends of a nucleic acid molecule, such as DNA or RNA. This activity is crucial for processes like DNA repair, replication, and degradation of RNA. Exonucleases can act on either the 5' or 3' ends of nucleic acids, and their specificity and directionality play vital roles in maintaining genetic integrity and regulating gene expression.
Heat and crupes
No, RNA polymerase does not have exonuclease activity during transcription.
RNA molecules that act as enzymes are called ribozymes(also called RNA enzymes or catalytic RNA).They have a well defined tertiary structure (like proteins), their activity is often coordinated to metal ions as cofactors, and they have been found to catalyze reactions in which a ribosome removes the amino group from an amino acid.
Nucleases catalyze the hydrolysis of phosphodiester bonds in nucleic acids, resulting in the cleavage of DNA or RNA molecules. This enzymatic activity allows nucleases to degrade or fragment nucleic acids.
An autophosphorylation is the phosphorylation of a kinase protein catalyzed by its own enzymatic activity.
Enzymes are proteins.Note: RNA can also produce enzymatic reactions, but they are called ribozymes.
Enzymatic activity is demonstrated by the ability of enzymes to catalyze biochemical reactions. This can be observed by changes in substrate concentration, product formation, or by measuring activity using specific assays such as spectrophotometry or mass spectrometry. Additionally, enzyme activity can be modulated by factors such as pH, temperature, and the presence of cofactors or inhibitors.
Refrigeration is not applicable to preserve sample for enzymatic assay because enzymes may lose their activity at extremely low temperatures as well. This may account for storing enzymes at 5° C or below without affecting the enzymatic activity permanently. (Anubhav, 2012)
Proteins are the primary macromolecules that can act as enzymes. They catalyze biochemical reactions by lowering the activation energy needed for the reaction to occur. Other macromolecules like RNA can also exhibit enzymatic activity, known as ribozymes.