If there is a EcoR1 site in either the middle of the Glo gene, or in the middle of the selectable marker site in the plasmid, it would likely disable either Glo, or the plasmid.
If a plasmid is cut at more than one site by restriction enzymes, it would result in multiple DNA fragments. These fragments can be ligated back together in different combinations, resulting in plasmids with different sizes or configurations. This can lead to the creation of recombinant plasmids with altered properties compared to the original plasmid.
Jellyfish don't bite... they sting.
It would become fragments of DNA and no more the plasmid will be in circular form.
If the plasmid were cut at more than one site, it could result in the fragmenting of the plasmid into smaller pieces. This could lead to difficulties in maintaining the integrity of the plasmid during cloning processes, affecting the stability and functionality of the plasmid. Additionally, it may disrupt the insertion of foreign DNA or hinder the replication of the plasmid in host cells.
The sting will burn
They are said to be denatured.Their tritiary structure is lost.
youd kill it
The enzyme will be re-used in another reaction.
dfdf
You'd probably get stung.
Water would diffuse into the cells of the jellyfish, causing it to bloat up and possibly burst.
If you're caught, depending on what the restriction is, you could be fined, and even have your licence suspended.