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It depends on why you want to use it. In most cases, depositions are inadmissible hearsay, and cannot be introduced as evidence at trial. However, if you want to impeach a witness on cross examination, the transcript could be used for that, regardless of what the prior case was about. Also, if there is a situation where the witness is unavailable for trial, you may be able to get the transcript in under certain circumstances.

If by "use the transcript" you mean read it and use it in the investigation process to build your case, then the answer is yes.

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